Products / Application / Genome Editing
Products
Programmable Nucleases
- Ideal for direct introduction of Cas9/sgRNA complexes
- Dual NLS for improved transport to the nucleus
- Compatible with EnGen® sgRNA Synthesis Kit, S. pyogenes and the EnGen Mutation Detection Kit
More Information:
- Product Overview
- Genome Editing brochure - tools for CRISPR/Cas applications
sgRNA Synthesis Reagents and Kits
- Rapid generation of microgram quantities of sgRNA
- Complete workflow of less than one hour
- Simple protocol requires only a user-supplied ~55 nucleotide ssDNA target-specific oligonucleotide
- Compatible with EnGen Spy Cas9 NLS, EnGen Spy Cas9 Nickase, EnGen Spy dCas9 (SNAP-tag®)
More Information:
Mutation Detection Reagents and Kits
- Simple protocol for detecting targeting efficiency in genome editing experiments
- Optimized reagents for performing robust T7 Endonuclease-based detection of genome editing events
- Q5® Hot Start High Fidelity 2X Master Mix included for robust amplification, high fidelity and convenience
- Rapid protocol requires no cleanup between PCR and digestion
More Information:
- Product Overview
- Genome Editing brochure - tools for CRISPR/Cas applications
CRISPR screening
- Standard Oligo Pools
- ssDNA delivered in a pool
- up to 300nt
- Cloned Oligo Pools
- Plasmid DNA in a pool
- Up to 250nt
- Cloned into a vector of your choice
- High Accuracy - 1:3000nt low error rate, Precision Editing of Target Loci
- High Uniformity - Maximize Screening Efficiency
- Oligo Lengths Up to 300nt - More Space for Innovation
More Information:
- Oligo Pools resources
- Publication: Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell. 2021;184(9):2503-2519.e17. doi:10.1016/j.cell.2021.03.025
