Products / Application / RNA Analysis
Products
Total RNA Extraction
- For use with blood, cells and tissues, saliva, and buccal/nasopharyngeal swabs
- Also works with tough to lyse samples (bacteria, yeast, plant)
- Effectively purifies total RNA of all sizes, including small RNAs >20 nt
- Generate RNA suitable for RT-qPCR, RT-PCR, and RNA-seq libraries
RNA Isolation & Enrichment
- Superior depletion of rRNA from human, mouse, and rat RNA
- Depletion of cytoplasmic and mitochondrial rRNA
- Compatible with a broad range of input amounts: 10 ng - 1 µg
- Suitable for low-quality or high-quality RNA
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
More Information:
- NEBNext Selector – to determine recommended products and protocols
RNA Library Prep for Illumina
- Generate the highest yields of high-quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low-quality RNA, including FFPE
cDNA synthesis
- Less than 15-minute first-strand cDNA synthesis protocol
- Combine with Luna qPCR master mixes for robust RT-qPCR results
- Eliminate pipetting errors with non-interfering, visible tracking dye
- Single-tube supermix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
More Information:
- cDNA synthesis – application overview
- FAQ: How do I choose between LunaScript® RT SuperMix Kit and ProtoScript first strand cDNA synthesis kits?
RNA synthesis
- An extremely flexible system for in vitro transcription of RNA using T7 RNA Polymerase
- Up to 180 μg of RNA per reaction from 1 μg of control template
- Enables full substitution of NTPs for labeling and incorporation of modified bases
- Linearized control template included for verification of RNA synthesis
More Information:
