Products / Application / Epigenetics
Products
Library Preparation Kits
Superior to bisulfite sequencing, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high-quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.
- Superior sensitivity of detection of 5-mC and 5-hmC
- Greater mapping efficiency
- More uniform GC coverage
- Detection of more CpGs with fewer sequence reads
- Uniform dinucleotide distribution
- High-efficiency library preparation, with larger library insert sizes
- Conversion module also available separately
More Information:
- NEBNEXT® Enzymatic Methyl-seq Workflow – Featured video
- Technical Note – extensive performance data
Target Enrichment Solutions
NGS panels are using dsDNA probes for hybridization capture-based target enrichment. This panel targets 3.98M CpG sites through 123 Mb of genomic content to target biologically relevant methylation markers. Expansive content makes this panel an ideal choice for investigators to explore the methylation fraction in a diverse range of applications from cancer metastasis, human development, and functional genomics.
Why choosing us?
- Hybridization capture-based dsDNA probes: allows high sensitivity
- Proprietary design algorithms: design probes in order to balance the coverage across difficult-to-sequence or difficult-to-map regions
- Proprietary silicon-based DNA synthesis platform: allows industrialized the production of cost-effective, high-fidelity and high-throughput DNA
More Information:
- Discovery in methylation profiling – Product sheet
Methylated DNA Enrichment Kit
Selectively enriching double-stranded methyl-CpG DNA from fragmented genomic DNA.
- High-affinity binding provides greater sensitivity.
- Elution in a small volume simplifies downstream applications.
- Easy-to-use protocol yields enriched fractions in less than 2 hours.
- Enriched methylated DNA fractions can be easily ligated to double-stranded adaptors for Next Generation Sequencing.
- Highly pure product from a wide range of input DNA concentrations.
More Information:
5-hmC and 5-mC Identification and Quantification
- Discrimination between 5-mC and 5-hmC in CCGG sequences using enzymatic digestion and PCR amplification
- Relative quantitation of 5-mC and 5-hmC
- Easy-to-use protocol
More Information:
- Epigenetics Brochure – Understanding histone & DNA modification
RNA Methylation Enrichment
Enrich m6A modified RNA in immunoprecipitation protocols for downstream analysis by next-generation RNA sequencing or RT-qPCR.
- Complete protocol for enrichment of m6A-modified –RNA and analysis by RT- qPCR included
- RNA controls (m6A modified and unmodified RNA) enable monitoring of enrichment and depletion
- Antibody supplied in a ready-to-use solution form
More Information:
- Epigenetics Brochure – Understanding histone & DNA modification
