Twist Variant Libraries | BioArrow Technology Ltd.

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Twist Variant Libraries

	Site Saturation Library	Spread Out Low Diversity Library	Combinatorial Variant Library	Combinatorial Assembly Library
What we can offer?	Single codon substitution per variant	Multiple codon substitutions scattering along the WT sequence (< 1.000bp) per variant	Multiple codon substitutions in a single/ multiple stretches (~100bp, up to 6 domains) per variant	Multiple variant domains (gene fragments) assembled to form ~5kb long insert at up to 10,000 combinations
What can it apply to?	To identify key residues in protein structure and function	To explore the sequence space encoded by mutating multiple positions simultaneously scattered across the parental sequence to generate a diverse library of proteins	To construct complex, high diversity libraries at confined regions (for example, CDRs)	To identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multi-specific VHH libraries
Examples of application	Enzymatic performance enhancement Signal transduction Growth factor binding affinity optimization Antibody binding site modification Improvement in expression in difficult host types Protein robustness/stress tolerance Promoter optimization Single base exploration for genetic element factor optimization Transcription modification
Why choose us?	More precise than traditional methods, e.g. NNK, TRIM and epPCR Complete control over codon usage (all 64 codons available) Verified uniformity of variant representation by NGS NGS verification of modified regions
How it works?	Send your enquiry to sales@bioarrow.com

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