Products / Application / DNA Amplification, PCR & qPCR
Products
High-fidelity PCR
- Highest fidelity amplification (~280X higher than Taq)
- Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Also available: Q5 Hot Start standalone polymerase and hot start master mix formats
More Information:
- Q5® High-Fidelity DNA Polymerase brochure – The finest in fidelity for over 10 years
- PCR Selector – tools to determine recommended products and protocols
Quantitative PCR (qPCR)
- Rapid, sensitive, and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences
- Offers exceptional sensitivity, reproducibility, and qPCR performance
- Non-interfering, blue tracking dye eliminates pipetting errors
- Contains a universal passive reference dye that enables compatibility with various instrument platforms
More Information:
- Luna® Universal qPCR & RT-qPCR product overview
- Brochure
Isothermal Amplification
- Provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets.
- Reaction temperature: 65°C
- Amplicon size: < 250nt
- Compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.
- This reagent has been incorporated into Oxford Nanopore’s LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.
More Information:
- Loop-Mediated Isothermal Amplification product overview
- NEB LAMP Primer Design Tool – to design core and loop primers for your LAMP reactions
Long Range PCR
- Optimized enzyme blend for amplification up to 30 kb
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates
More Information:
PCR Automation
Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows, particularly for automated reaction set-up. This innovative solution offers excellent precision, outstanding troughput and increased walkaway times.
Simply faster and more precise
Three independent, task specific arms move in parallel to simultaneously and efficiently automate methods. Sample preparation, labware movement to peripheral devices and plate-to-plate or reagent additions can all occur in parallel — maximizing performance
Save reagents and samples
Adaptive Signal Technology™, a new generation of liquid level sensing, enables the use of smaller sample volumes, minimizes the risk of errors and provides exceptional automated error handling
Simpler workflow design with FluentControl™ and Smart Commands
Device integration options including:
- 1/2D barcode scanners
- Temperature-controlled devices
- Plate sealer
- Inheco On-Deck Thermocycler integration
More Information:
